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Seeds from forty eight maternal samples throughout 5 Arkansas rice fields have been collected during the harvest season of 2018. US weedy rice lacks geographical genetic structure4,29, so this sampling could also be considered representative of the southern US rice production region4,22,29. 40× common coverage) were generated using leaf tissue from one seed per maternal plant grown to the seedling stage. 19.34 million SNPs. Previously revealed genomes included 22 historic weedy (11 SH and eleven BHA), 49 cultivated (10 aus, 5 aromatic, 12 indica, 12 temperate japonica, and 10 tropical japonica) and 27 wild rice accessions. To evaluate the general genetic composition of contemporary weed samples compared to historic US weed strains, we employed principal part analysis (PCA) and ADMIXTURE evaluation. The PCA revealed relatively tight inside-pressure grouping of cultivated and historic (pre-2000) weedy rice, with contemporary weedy rice showing a much broader dispersion (Fig. 1). PC1 (22.8% variation defined) separated the japonica and indica subspecies lineages, which is the deepest divergence within the Asian rice taxonomy.
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